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Gilberto Guimarães Villela

Gilberto Guimarães Villela, Brazilian biochemist and microbiologist (Lavras, Minas Gerais State 12 July 1904 – Rio de Janeiro 17 July 1977)
 
Discovered the activation of liver xanthine oxidase with carbon tetrachloride

With A.M. Calcagnotto. Studies on the toxicity and absorption of a new chelate of aluminium and calcium. Rev. Bras. Pesq. Med. Biol. 6(3-4):165-9, 1973


Proposed a new interpretation of oedema formation in hookworm anemia in Mem. Inst. Oswaldo Cruz 6:55, 1929


DEVELOPED NEW TECHNIQUES/METHODS
With M. Bacila. A rapid and sensitive method for the determination of xanthine oxidase activity using the oxygen electrode. Nature 184 (Suppl. 18):1394-5, 1959
 

A colorimetric method for assaying xanthine dehydrogenase activity

Determination of rat liver xanthine dehydrogenase by triphenyltetrazolium chloride. Rev. Bras. Biol. 14:455-8, 1954 

With A. Cury. A microbiological assay method for biotin. Proc. Soc. Exp. Biol. Med. 76(2):341-3, 1951

A new technique for isolation of the snake erythrocyte nuclei using tyrothricin

Isolation and properties of snake erythrocyte nuclei. Exp. Biol. Med. 66(2):398-400, 1947

Um novo “test” ponderal para avaliação da atividade do hormônio cortical. Mem. Inst. Oswaldo Cruz 38(2):173-6, 1943

A technique for isolation of active protein fraction of lepromine

Sur la fraction active de l’antigene de Mitsuda. Bull. Soc. Franc. Dermat. Syph. p.46, 1939

With A.M. Leal. A new color reation of vitamin B (thiamin, aneurin). Science 90 (2330):179, 1939

Técnica simples e precisa para dosagem dos protídeos totais no plasma. O Hospital 8:5, 1936

Micro-extractor para dosagem do cholesterol no sangue. Mem. Inst. Oswaldo Cruz 27(1):17-20, 1933

 

With M.I. de Mello. Um novo test para a avaliação da atividade biológica do princípio antianemico do fígado

 

A method for determination of dehydrogenase activity

Gilberto Villela technique for determination of urinary ascorbic acid rate (a modification of Harris-ray method)

Villela reaction for determination of nicotinamide in blood


Papers in Nature

With R.R. Abreu & L.A. Abreu. Mucoids of some micro-organisms. Nature 196:582, 1962

With O.R. Affonso & E. Mitidieri. Effect of colchicine on rat blood serum xanthine oxidase. Nature 193:64, 1962

With O.R. Affonso & E. Mitidieri. Effect of colchicine on rat liver xanthine oxidase. Nature 192:666-7, 1961

Quinine oxidase in liver and blood plasma of rabbits poisoned with carbon tetrachloride. Nature 190:807-8, 1961

With M. Bacila. A rapid and sensitive method for the determination of xanthine oxidase activity using the oxygen electrode. Nature 184 (Suppl 18):1394-5, 1959

With O.R. Affonso, E. Mitidieri & L.P. Ribeiro. Detection of xanthine dehydrogenase activity in soluble proteins of rat liver separated by paper electrophoresis. Nature 178 (4531):492-3, 1956

Carotenoids of some Brazilian freshwater gastropods of the genus Pomacea. Nature 178 (4524):93, 1956

With E. Mitidieri & O.R. Affonso. Intracellular distribution of xanthine oxidase in the rat liver. Nature 175 (4468):1087, 1955

With E. Mitidieri. Rat liver xanthine oxidase in carbon tetrachloride poisoning. Nature 175 (4448):208-9, 1955

Ribosenucleic acid in snake erythrocyte nuclei. Nature 164 (4172):667, 1949

Papers in Science

With A. Cury. On the pigments of Allescheria boydii. Science 112 (2911):430-1, 1950

With H.H. Anderson, E.L. Hansen & R.K. Reed. Some physical and biologic proteins of subtilin and other antibiotics. Science 103 (2675):419-20, 1946

Vitamin C in the needles of some conifers. Science 99 (2563):125, 1944

With A.M. Leal. A new color reaction of vitamin B1 (thiamin, neurin). Science 90 (2330):179, 1939

Vitamin B1 in cerebrospinal fluid. Science 89 (2307):251, 1939