Demonstrated the specifity of anti snake serum (1899)

With Bruno Rangel Pestana prepared anti Elaps serum and noted that species presenting great  sensibility to venoms are that which produce more antibodies (1910)

First to develop anti scorpion serum (1908)

First to introduce polivalents sera   

With Jean Vellard showed the role of lipoids in immunology (1927) and developed anti arachnid serum (1925-6)

Introduced the concentration process of sera by ammonium sulfate (later replaced by sodium sulfate)

Indicated the potency of serum in venom milligrams that each cubic centimeter neutralizes (1907)

Established evaluation and antitoxic activity of anti venomous sera method (adopted in Brazil, Argentina and other countries)

Described the action of heterologous antibodies

Described coagulant, anticoagulant, agglutinant, cytotoxic, hemolytic, proteolytic properties of many snake venoms and noted the distinct sensibility of erythrocyte in many species facing the same poison

With Argentine herpetologist Avelino Barrio discovered Veratrine properties of poison from Crotalus durissus (1950s)

Discovered ophiophagous features of  muçuarana (Clelia sp.) in 1910

With US researcher Monoelesser performed studies about the action of snake venom on leprosy (1920s)

Dosagem do valor anti-tóxico do serum anti-peçonhento. Rev. Med. S. Paulo 10(22):457-62, 1907 

With J. Vellard. Imunização por via intradérmica e por via subcutânea pelo soro antictenus. Método de dosagem e primeira aplicação no homem. Brazil Médico 31(2):224-7, 1925

Rosenfeld, G. Vital Brazil como pesquisador cientifico. Ciencia e Cultura 17(2), 1965  (biliography)